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Ticket #84 (closed enhancement: moved)

Opened 2007-03-23T13:29:20-05:00

Last modified 2014-01-09T13:38:52-06:00

Use system response file to calibrate data via deconvolution

Reported by: curtis Owned by: nor
Priority: critical Milestone:
Component: slim-plotter Severity: serious
Keywords: Cc:
Blocked By: Blocking:

Description (last modified by curtis)

We collect a system response file that represents the instrument's behavior when no excitation takes place. This file should be used to adjust the lifetime histograms (via deconvolution) to improve the quality of the fits.

Change History

comment:1 Changed 2007-10-09T09:16:27-05:00 by curtis

  • Description modified
  • Summary changed from Use system response file to calibrate exponential curve fits to Use system response file to calibrate data via deconvolution

comment:2 follow-up: ↓ 4 Changed 2007-10-09T09:22:25-05:00 by curtis

There are actually a total of three types of corrections to do:

  1. The deconvolution for each channel's lifetime histogram.
  2. A deskew of the spectral channel delays based on values in the calibration file.
  3. A scaling factor to apply to each spectral channel, to adjust for an "intensity skew" across channels.

The system response file will contain all of this information, once the engineers figure out the most reliable way to collect it.

comment:3 Changed 2007-10-09T09:22:57-05:00 by curtis

  • Priority changed from minor to critical

comment:4 in reply to: ↑ 2 Changed 2007-10-09T09:53:02-05:00 by curtis

Replying to curtis:

Ticket #135 has more details on the spectral calibration issue.

Also, the current plan is to embed relevant calibration information into each dataset, rather than have separate calibration files, to ensure the correct calibration is always available; see ticket #183.

comment:5 Changed 2007-10-09T09:53:15-05:00 by curtis

  • Owner curtis deleted

comment:6 Changed 2007-10-09T11:09:31-05:00 by curtis

Be sure to check out the  Parallel Spectral Deconvolution 2D and 3D plugin for ImageJ, as it may be relevant.

comment:7 Changed 2007-10-09T12:43:18-05:00 by curtis

  • Owner set to nor

comment:8 Changed 2014-01-09T13:38:52-06:00 by curtis

  • Status changed from new to closed
  • Resolution set to moved
  • Severity set to serious

See  https://github.com/uw-loci/slim-plotter/issues/4